4JXJ
Crystal Structure of Ribosomal RNA small subunit methyltransferase A from Rickettsia bellii Determined by Iodide SAD Phasing
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-27 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 80.380, 81.330, 38.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.2124 |
Rwork | 0.210 |
R-free | 0.26490 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.510 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 8.940 | 2.050 |
High resolution limit [Å] | 2.000 | 6.320 | 2.000 |
Rmerge | 0.090 | 0.049 | 0.370 |
Number of reflections | 32726 | 671 | 2226 |
<I/σ(I)> | 14.52 | 31.85 | 3.45 |
Completeness [%] | 99.4 | 99.7 | 92.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | Wizard3/4(F6): 25% PEG-1500, 0.1M SPG Buffer, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |