4JXJ

Crystal Structure of Ribosomal RNA small subunit methyltransferase A from Rickettsia bellii Determined by Iodide SAD Phasing

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-27
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	80.380, 81.330, 38.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.000
R-factor	0.2124
Rwork	0.210
R-free	0.26490
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.510
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	8.940	2.050
High resolution limit [Å]	2.000	6.320	2.000
Rmerge	0.090	0.049	0.370
Number of reflections	32726	671	2226
<I/σ(I)>	14.52	31.85	3.45
Completeness [%]	99.4	99.7	92.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	289	Wizard3/4(F6): 25% PEG-1500, 0.1M SPG Buffer, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K