4JWY
GluN2D ligand-binding core in complex with propyl-NHP5G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Wavelength(s) | 1.10 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 59.996, 113.899, 95.695 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.456 - 2.000 |
| R-factor | 0.1929 |
| Rwork | 0.191 |
| R-free | 0.23320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3oen |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.172 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 22209 |
| Completeness [%] | 98.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 100 mM CAPS, 3.5-4.5 M sodium formate, 8-12% 1,4-butanediol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
