4JWX
GluN2A ligand-binding core in complex with propyl-NHP5G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Wavelength(s) | 1.10 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 52.031, 52.031, 198.823 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.184 - 1.500 |
R-factor | 0.1615 |
Rwork | 0.160 |
R-free | 0.19460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ftj |
RMSD bond length | 0.007 |
RMSD bond angle | 1.145 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.500 |
Number of reflections | 44489 |
Completeness [%] | 98.4 |
Redundancy | 12.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 100 mM HEPES-NaOH, 10-18% PEG8000, 100 mM calcium acatate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |