4JV8

The crystal structure of PDE6D in complex with rac-S1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-03-21
Detector	PSI PILATUS 6M
Wavelength(s)	0.9786
Spacegroup name	P 32 2 1
Unit cell lengths	55.970, 55.970, 115.560
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.980 - 1.450
R-factor	0.1814
Rwork	0.180
R-free	0.20862
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3t5g
RMSD bond length	0.032
RMSD bond angle	2.649
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0093)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.980	1.500
High resolution limit [Å]	1.450	1.450
Number of reflections	37973
Completeness [%]	99.9	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	100mM Sodium Acetate(pH4.6) 2.0M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 293K