4JUQ
Pseudomonas aeruginosa MetAP T2N mutant, in Mn form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Detector technology | CCD |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.593, 105.599, 133.917 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.870 - 2.200 |
R-factor | 0.1812 |
Rwork | 0.179 |
R-free | 0.22930 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.762 |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 36.870 |
High resolution limit [Å] | 2.200 |
Number of reflections | 58806 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop vapor diffusion | 7.5 | 100 mM HEPES, 70% MPD, pH 7.5, hanging drop vapor diffusion |