4JTA

Crystal structure of Kv1.2-2.1 paddle chimera channel in complex with Charybdotoxin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	P 4 21 2
Unit cell lengths	144.400, 144.400, 284.103
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.500
Rwork	0.210
R-free	0.23580
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.075	0.043	0.440
Number of reflections	99907
<I/σ(I)>	15.3
Completeness [%]	96.1	99.7	71.1
Redundancy	6.9	8.2	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.9	293	PEG 400, POTASSIUM CHLORIDE, TRIS , pH 8.9, vapor diffusion, hanging drop, temperature 293K