4JSV
mTOR kinase structure, mechanism and regulation.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2011-12-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97925 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 139.400, 163.200, 207.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 100.000 - 3.500 |
R-factor | 0.2314 |
Rwork | 0.231 |
R-free | 0.25080 |
Structure solution method | MIR |
RMSD bond length | 0.022 |
RMSD bond angle | 1.156 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 3.630 |
High resolution limit [Å] | 3.500 | 7.540 | 3.500 |
Rmerge | 0.185 | 0.048 | 0.665 |
Number of reflections | 57899 | ||
<I/σ(I)> | 3.8 | ||
Completeness [%] | 94.6 | 95.2 | 83.6 |
Redundancy | 3.6 | 4.4 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop vapor diffusion | 8.5 | 277 | PEG 8000, NaCl, pH 8.5, hanging drop vapor diffusion, temperature 277K |