4JS1
crystal structure of human Beta-galactoside alpha-2,6-sialyltransferase 1 in complex with cytidine and phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-11-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 61 |
Unit cell lengths | 65.292, 65.292, 162.232 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.387 - 2.090 |
R-factor | 0.1953 |
Rwork | 0.193 |
R-free | 0.22820 |
Structure solution method | SIRAS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.252 |
Data reduction software | XDS |
Data scaling software | SADABS |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine: dev_1327)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.400 | 2.160 |
High resolution limit [Å] | 2.090 | 2.090 |
Number of reflections | 22378 | |
<I/σ(I)> | 13.5 | 1.3 |
Completeness [%] | 99.4 | 99.4 |
Redundancy | 9.9 | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 294 | 0.1M MES/NaOH, pH 5.8, 20% PEG 2000 MME, 0.01M CaCl2, 0.01M MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 294K |