4JRP
Structure of E. coli Exonuclease I in complex with a 5cy-dT13 oligonucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-05-05 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 43 |
| Unit cell lengths | 91.300, 91.300, 159.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.580 - 1.950 |
| R-factor | 0.21543 |
| Rwork | 0.213 |
| R-free | 0.25909 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fxx |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.302 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.600 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.078 | 0.363 |
| Number of reflections | 89174 | |
| <I/σ(I)> | 8.4 | 2 |
| Completeness [%] | 99.2 | 93.9 |
| Redundancy | 3 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 5 % 2-propanol, 25% glycerol, 1.2 M ammonium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
