4JRB
Structure of Cockroach Allergen Bla g 1 Tandem Repeat as a EGFP fusion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-BM-B |
| Synchrotron site | APS |
| Beamline | 23-BM-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 91.768, 141.920, 93.465 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.188 - 2.414 |
| R-factor | 0.2001 |
| Rwork | 0.198 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ai4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.080 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.089 | 0.458 |
| <I/σ(I)> | 14.9 | 2.5 |
| Completeness [%] | 99.3 | 94 |
| Redundancy | 7.1 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 100mM Tris, 10% propanol, 20% PEG4K, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
