4JQU

Crystal structure of Ubc7p in complex with the U7BR of Cue1p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-23
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	46.810, 48.463, 95.734
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.467 - 1.810
R-factor	0.1863
Rwork	0.184
R-free	0.22470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ucz
RMSD bond length	0.005
RMSD bond angle	0.974
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.870
High resolution limit [Å]	1.810	3.900	1.810
Rmerge	0.063	0.035	0.494
Number of reflections	20415
<I/σ(I)>	27	39.2	3.4
Completeness [%]	98.7	99.6	93.7
Redundancy	7.5	7.5	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	25% PEG3350, 0.2 M ammonium acetate, 0.1 M Bis-Tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K