4JQ0

Voltage-gated sodium channel 1.5 C-terminal domain in complex with FGF12B and Ca2+/calmodulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 31 2 1
Unit cell lengths	115.200, 115.200, 120.110
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.570 - 3.840
R-factor	0.2571
Rwork	0.253
R-free	0.31990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.979
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX ((AutoMR: 1.8_1069))
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.068	50.000	3.870
High resolution limit [Å]	3.830	10.290	3.800
Number of reflections	3705
Completeness [%]	40.6	98.3	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	290	20% PEG3350, 0.18 M MgSO4, 0.1 M sodium iodide, and CaCl2, pH 7.5, EVAPORATION, temperature 290K