4JP5

X-ray structure of uridine phosphorylase from Yersinia pseudotuberculosis in unliganded state at 2.27 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-12-05
Detector	PSI PILATUS 6M
Wavelength(s)	0.8266
Spacegroup name	P 32
Unit cell lengths	93.750, 93.750, 146.580
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.860 - 2.270
R-factor	0.1792
Rwork	0.178
R-free	0.20210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dps
RMSD bond length	0.005
RMSD bond angle	0.904
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.860	48.860	2.450
High resolution limit [Å]	2.270	30.000	2.270
Rmerge	0.123	0.023	0.928
Rmeas		0.028	1.052
Number of reflections	65380	21	12462
<I/σ(I)>	11.71	43.48	1.5
Completeness [%]	98.1	70	91.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	10mM Tris HCl, 0.1M Tris-maleate-NaOH,20% (w/v) PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K