4JP4
Mmp13 in complex with a reverse hydroxamate Zn-binder
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97910 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.616, 36.130, 95.755 |
| Unit cell angles | 90.00, 131.02, 90.00 |
Refinement procedure
| Resolution | 51.160 - 1.430 |
| R-factor | 0.1525 |
| Rwork | 0.151 |
| R-free | 0.17550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.476 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 54.000 |
| High resolution limit [Å] | 1.430 |
| Number of reflections | 62827 |
| Completeness [%] | 95.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 27.5% PEG 4000, 1.25M AmFormate, 100mM TrisHCl pH 8.5 , VAPOR DIFFUSION, HANGING DROP, temperature 292K |
