4JMX

Structure of LD transpeptidase LdtMt1 in complex with imipenem

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-31
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97
Spacegroup name	P 43 21 2
Unit cell lengths	67.397, 67.397, 119.564
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.950 - 2.550
R-factor	0.16444
Rwork	0.161
R-free	0.23317
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.403
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.550	2.550
Number of reflections	9506
Completeness [%]	99.8	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	protein concentration of 3.5 mg/mL and 1.24 M sodium phosphate and 0.16 M potassium phosphate, 10 mM TCEP, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K