4JMH
Crystal structure of synthetic protein in complex with double pY peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Detector technology | CCD |
Collection date | 2012-07-28 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03316 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 89.853, 92.190, 59.596 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.173 - 2.408 |
R-factor | 0.2158 |
Rwork | 0.213 |
R-free | 0.27560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jmg |
RMSD bond length | 0.009 |
RMSD bond angle | 1.372 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.400 |
Number of reflections | 9059 |
<I/σ(I)> | 21.8 |
Completeness [%] | 100.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.66% ammonium sulfate, 2.32% PEG400, 0.1 M HEPES , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |