4JKF
Open and closed forms of T1791P+R1865A human PRP8 RNase H-like domain with bound Mg ion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1158 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.171, 77.253, 93.601 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.030 - 1.950 |
R-factor | 0.1886 |
Rwork | 0.186 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3enb |
RMSD bond length | 0.011 |
RMSD bond angle | 1.345 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC (5.6.0117) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.020 |
High resolution limit [Å] | 1.950 | 4.200 | 1.950 |
Rmerge | 0.054 | 0.018 | 0.528 |
Number of reflections | 38416 | ||
<I/σ(I)> | 13 | ||
Completeness [%] | 94.3 | 98.9 | 99.8 |
Redundancy | 4 | 3.9 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 298 | 15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K |