4JKA

Open and closed forms of R1865A human PRP8 RNase H-like domain with bound Co ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-02
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	76.249, 78.223, 94.373
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.130 - 1.320
R-factor	0.1431
Rwork	0.142
R-free	0.16580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3enb
RMSD bond length	0.010
RMSD bond angle	1.375
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	REFMAC (5.6.0117)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.370
High resolution limit [Å]	1.320	2.840	1.320
Rmerge	0.045	0.035	0.390
Number of reflections	126457
<I/σ(I)>	20.5
Completeness [%]	95.2	99.4	85.3
Redundancy	4.3	5.2	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K