4JK8
Open and closed forms of R1865A human PRP8 RNase H-like domain with bound Mg ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.198, 78.037, 94.063 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.290 - 1.150 |
| R-factor | 0.1411 |
| Rwork | 0.140 |
| R-free | 0.15970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3enb |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.508 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC (5.6.0117) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.170 |
| High resolution limit [Å] | 1.150 | 3.120 | 1.150 |
| Rmerge | 0.036 | 0.031 | 0.481 |
| Number of reflections | 196125 | ||
| <I/σ(I)> | 20.6 | ||
| Completeness [%] | 98.9 | 98.9 | 96.6 |
| Redundancy | 4.2 | 4.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | 15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K |
