4JJZ
Crystal Structure of N10-Formyltetrahydrofolate Synthetase with ADP and Formylphosphate
Replaces: 3RBOExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-11 |
Detector | SBC-1 |
Wavelength(s) | 1.59 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 91.170, 212.976, 53.444 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.810 - 2.500 |
R-factor | 0.18459 |
Rwork | 0.181 |
R-free | 0.25071 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.709 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 | 2.590 |
High resolution limit [Å] | 2.500 | 2.590 | 2.500 |
Rmerge | 0.085 | ||
Number of reflections | 37221 | ||
<I/σ(I)> | 8 | ||
Completeness [%] | 91.5 | 78.9 | 64.8 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 278 | 75 mM Potassium Maleate Buffer pH 7.6, 1 mM dithiothreitol, 40% Ammonium Sulfate, 2% PEG 1000, vapor diffusion, hanging drop, temperature 278K |