4JJZ

Crystal Structure of N10-Formyltetrahydrofolate Synthetase with ADP and Formylphosphate

Replaces:  3RBO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-11
Detector	SBC-1
Wavelength(s)	1.59
Spacegroup name	P 21 21 2
Unit cell lengths	91.170, 212.976, 53.444
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.810 - 2.500
R-factor	0.18459
Rwork	0.181
R-free	0.25071
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.709
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	2.690	2.590
High resolution limit [Å]	2.500	2.590	2.500
Rmerge	0.085
Number of reflections	37221
<I/σ(I)>	8
Completeness [%]	91.5	78.9	64.8
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	278	75 mM Potassium Maleate Buffer pH 7.6, 1 mM dithiothreitol, 40% Ammonium Sulfate, 2% PEG 1000, vapor diffusion, hanging drop, temperature 278K