4JJR

A P21 crystal form of mammalian casein kinase 1 delta

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-26
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 1 21 1
Unit cell lengths	52.145, 102.251, 64.593
Unit cell angles	90.00, 101.53, 90.00

Refinement procedure

Resolution	40.665 - 2.408
R-factor	0.2115
Rwork	0.210
R-free	0.23710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3UZP CHAIN A
RMSD bond length	0.004
RMSD bond angle	0.962
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.5.0)
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.470
High resolution limit [Å]	2.400	5.490	2.400
Rmerge	0.094	0.044	0.681
Number of reflections	25594
<I/σ(I)>	6.2
Completeness [%]	100.0	99.8	100
Redundancy	7.7	7.5	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	7	298	100 mM succinic acid, 15% PEG 3350, pH 7.0, hanging drop, temperature 298K