4JJK

Crystal Structure of N10-Formyltetrahydrofolate Synthetase with Folate

Replaces:  3QB6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	160.948, 160.948, 256.532
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.750 - 3.000
R-factor	0.21404
Rwork	0.210
R-free	0.28624
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3QB6
RMSD bond length	0.011
RMSD bond angle	1.449
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.050
High resolution limit [Å]	3.000	8.130	3.000
Rmerge	0.132	0.063	0.335
Number of reflections	24612
<I/σ(I)>	7.2
Completeness [%]	95.2	90.8	97.8
Redundancy	5.9	4.9	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	278	50-75 mM Potassium Maleate Buffer pH 7.0-8.0, 1 mM dithiothreitol, 38-46% Ammonium Sulfate, 1-3.5% PEG 1000 or PEG 1450, vapor diffusion, hanging drop, temperature 278K