Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 160.948, 160.948, 256.532 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.750 - 3.000 |
| R-factor | 0.21404 |
| Rwork | 0.210 |
| R-free | 0.28624 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3QB6 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.449 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 8.130 | 3.000 |
| Rmerge | 0.132 | 0.063 | 0.335 |
| Number of reflections | 24612 | ||
| <I/σ(I)> | 7.2 | ||
| Completeness [%] | 95.2 | 90.8 | 97.8 |
| Redundancy | 5.9 | 4.9 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 278 | 50-75 mM Potassium Maleate Buffer pH 7.0-8.0, 1 mM dithiothreitol, 38-46% Ammonium Sulfate, 1-3.5% PEG 1000 or PEG 1450, vapor diffusion, hanging drop, temperature 278K |
