4JI1
Crystal Structure of 30S ribosomal subunit from Thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 403.171, 403.171, 173.325 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.647 - 3.144 |
R-factor | 0.1667 |
Rwork | 0.165 |
R-free | 0.20700 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2vqe |
RMSD bond length | 0.007 |
RMSD bond angle | 1.212 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX (dev_1119) |
Refinement software | PHENIX (dev_1119) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.000 | 35.000 | 3.250 |
High resolution limit [Å] | 3.140 | 6.750 | 3.140 |
Rmerge | 0.069 | 0.021 | 0.627 |
Number of reflections | 241842 | ||
<I/σ(I)> | 13.1 | ||
Completeness [%] | 99.0 | 95.4 | 97.7 |
Redundancy | 3.4 | 3.3 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | MPD , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |