4JHW

Crystal Structure of Respiratory Syncytial Virus Fusion Glycoprotein Stabilized in the Prefusion Conformation by Human Antibody D25

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	200
Detector technology	CCD
Collection date	2012-12-19
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 3
Unit cell lengths	152.286, 152.286, 152.286
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.237 - 3.600
R-factor	0.2157
Rwork	0.213
R-free	0.26710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3RRR AND 4JHA
RMSD bond length	0.003
RMSD bond angle	0.908
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.730
High resolution limit [Å]	3.600	3.600
Number of reflections	13898
Completeness [%]	99.6	99.3
Redundancy		5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	30% (w/v) PEG 400, 3.75% (w/v) PEG 3350, 0.1 M HEPES pH 7.5, and 1% (v/v) 1,2-butanediol, VAPOR DIFFUSION, HANGING DROP, temperature 293K