4JFA
Crystal Structure of Plasmodium falciparum Tryptophanyl-tRNA synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.176, 213.252, 101.952 |
| Unit cell angles | 90.00, 97.15, 90.00 |
Refinement procedure
| Resolution | 47.830 - 2.600 |
| R-factor | 0.192 |
| Rwork | 0.191 |
| R-free | 0.22850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.855 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.585 | |
| Number of reflections | 62582 | |
| <I/σ(I)> | 2.94 | |
| Completeness [%] | 94.4 | 91.3 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.09M NPS, 0.1M Bicin pH 8.5, 12.5% MPD, 12.4 PEG 1000, 12.5% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
