4JF7

Structure of the parainfluenza virus 5 (PIV5) hemagglutinin-neuraminidase (HN) ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	200
Detector technology	IMAGE PLATE
Collection date	2011-11-28
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97872
Spacegroup name	I 4
Unit cell lengths	194.390, 194.390, 185.979
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.364 - 2.502
R-factor	0.1663
Rwork	0.166
R-free	0.20680
RMSD bond length	0.011
RMSD bond angle	1.180
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.082	0.037	0.522
Number of reflections	118507
<I/σ(I)>	20.3
Completeness [%]	100.0	99.8	100
Redundancy	6.3	6.2	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	298	1.6 M ammonium sulfate, 0.1 M MES monohydrate pH 6.5, 10% v/v 1,4-dioxane, VAPOR DIFFUSION, SITTING DROP, temperature 298K