4JDZ

Structures of SdrD from Staphylococcus aureus reveal the molecular mechanism of how the cell surface receptors recognize their ligands

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-13
Detector	MAR225
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	74.410, 73.880, 90.030
Unit cell angles	90.00, 102.42, 90.00

Refinement procedure

Resolution	50.000 - 2.100
R-factor	0.187
Rwork	0.185
R-free	0.23150
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	DM
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	5.700	2.140
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.062
Number of reflections	55836
<I/σ(I)>	33

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	0.2M Calcium chloride dihydrate, 0.1M HEPES sodium(7.5), 20%(v/v) 2-Propanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K