4JCD
S268Y Variant of JC Polyomavirus Major Capsid Protein VP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-15 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 150.150, 96.180, 128.360 |
Unit cell angles | 90.00, 110.39, 90.00 |
Refinement procedure
Resolution | 44.700 - 2.000 |
R-factor | 0.16584 |
Rwork | 0.164 |
R-free | 0.20416 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nxg |
RMSD bond length | 0.009 |
RMSD bond angle | 1.355 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.000 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 114754 | |
<I/σ(I)> | 12.6 | 2.57 |
Completeness [%] | 99.4 | 98.6 |
Redundancy | 3.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES, 0.2 M KSCN, 12% PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |