4JAX
Crystal structure of dimeric KlHxk1 in crystal form X
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-19 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91841 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.810, 178.300, 216.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.602 - 2.260 |
R-factor | 0.2007 |
Rwork | 0.200 |
R-free | 0.23980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | chain A of PDB entry 3O1W |
RMSD bond length | 0.010 |
RMSD bond angle | 1.502 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.850 | 2.320 |
High resolution limit [Å] | 2.260 | 2.260 |
Number of reflections | 190903 | |
<I/σ(I)> | 24.25 | 3.84 |
Completeness [%] | 99.8 | 100 |
Redundancy | 5.2 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 1 microliter protein (7.8 mg/ml KlHxk1 in buffer (10 mM Tris, 1 mM EDTA, 1 mM DTT, 0.5 mM PMSF, pH 7.4)) + 1 microliter reservoir (2.2 M (NH4)2HPO4, 0.1 M Tris pH 8.5), micro seeding, VAPOR DIFFUSION, HANGING DROP, temperature 298K |