4J9L
Human DNA polymerase eta-DNA ternary complex: misincorporation G opposite T after a C at the primer 3' end (CA/G)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2010-01-05 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 |
Unit cell lengths | 98.430, 98.430, 81.960 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.414 - 1.850 |
R-factor | 0.1751 |
Rwork | 0.172 |
R-free | 0.20840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mr2 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.169 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.140 | 1.900 |
High resolution limit [Å] | 1.850 | 2.070 | 1.850 |
Rmerge | 0.101 | 0.349 | 0.803 |
Number of reflections | 38030 | ||
<I/σ(I)> | 14.61 | 5.33 | 2.01 |
Completeness [%] | 98.6 | 98.2 | 96.8 |
Redundancy | 3.8 | 3.85 | 3.12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG2K-MME, KCl, MES, pH 6.5, vapor diffusion, hanging drop, temperature 298K |