4J97
Crystal Structure of FGF Receptor 2 (FGFR2) Kinase Domain Harboring the Pathogenic Gain-of-Function K659E Mutation Identified in Endometrial Cancer.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-11 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 0.97906 |
Spacegroup name | P 1 |
Unit cell lengths | 70.584, 70.278, 85.499 |
Unit cell angles | 92.39, 112.19, 115.98 |
Refinement procedure
Resolution | 41.349 - 2.548 |
R-factor | 0.2024 |
Rwork | 0.200 |
R-free | 0.25680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pvy |
RMSD bond length | 0.003 |
RMSD bond angle | 0.625 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.548 | 2.548 |
Number of reflections | 41785 | |
<I/σ(I)> | 24.5 | 5.1 |
Completeness [%] | 97.1 | 84.5 |
Redundancy | 3.5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100mM HEPES pH7.5, 25% PEG 4000, 200mM (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |