4J8N
Aurora A Kinase Apo
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0088 |
Spacegroup name | P 31 |
Unit cell lengths | 83.783, 83.783, 171.777 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.949 - 3.135 |
R-factor | 0.1884 |
Rwork | 0.184 |
R-free | 0.23360 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.886 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.180 |
High resolution limit [Å] | 3.130 | 8.480 | 3.130 |
Rmerge | 0.059 | 0.030 | 0.404 |
Number of reflections | 23697 | ||
<I/σ(I)> | 18.4 | ||
Completeness [%] | 99.9 | 99.2 | 100 |
Redundancy | 6.2 | 6.1 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Hanging drop vapor diffusion | 7 | 298 | 20 mg/ml Aurora A protein, 10% Tacsimate, 20% (w/v) PEG3350, pH 7.0, Hanging drop vapor diffusion, temperature 298K |