4J8F
Crystal structure of a fusion protein containing the NBD of Hsp70 and the middle domain of Hip
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.8726 |
Spacegroup name | P 41 |
Unit cell lengths | 65.596, 65.596, 141.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.700 |
R-factor | 0.197 |
Rwork | 0.194 |
R-free | 0.26130 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.243 |
Data scaling software | SCALA (3.3.16) |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 65.596 | 48.098 | 2.790 |
High resolution limit [Å] | 2.650 | 8.370 | 2.650 |
Rmerge | 0.025 | 0.491 | |
Total number of observations | 2770 | 12853 | |
Number of reflections | 17407 | ||
<I/σ(I)> | 16.1 | 23.2 | 1.6 |
Completeness [%] | 99.9 | 98.7 | 99.8 |
Redundancy | 5.1 | 5 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 291 | 17 % PEG-3350 and 0.2 M NH4I, pH 7.4, vapor diffusion, hanging drop, temperature 291K |