4J7F
SET7/9Y335pAF in complex with TAF10 peptide and AdoHcy
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9786 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 83.046, 83.046, 95.524 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 71.920 - 1.590 |
R-factor | 0.20215 |
Rwork | 0.201 |
R-free | 0.21540 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.506 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.920 | 1.640 |
High resolution limit [Å] | 1.590 | 1.600 |
Number of reflections | 48193 | |
Completeness [%] | 94.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | Sodium citrate, imidazole, nickel chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |