4J75

Crystal Structure of a parasite tRNA synthetase, product-bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-15
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	P 21 21 2
Unit cell lengths	117.033, 189.473, 51.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.1944
Rwork	0.193
R-free	0.22250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j76
RMSD bond length	0.007
RMSD bond angle	1.165
Data reduction software	XDS
Data scaling software	Aimless (0.1.27)
Phasing software	PHASER (2.5.2)
Refinement software	REFMAC (refmac_5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.640	2.480
High resolution limit [Å]	2.400	2.400
Rmerge	0.171	0.010
Total number of observations		32129
Number of reflections	45354
<I/σ(I)>	12.5	2.2
Completeness [%]	99.2	98.2
Redundancy	7.3	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M sodium citrate, 20% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K