Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 107.297, 107.297, 81.290 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 61.182 - 2.520 |
| R-factor | 0.2271 |
| Rwork | 0.221 |
| R-free | 0.28570 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.357 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.200 | 2.650 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.127 | |
| Number of reflections | 9602 | |
| <I/σ(I)> | 32 | |
| Completeness [%] | 98.1 | 95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100mM TRIS pH 7.5-9.0,180-200 mM NaCl, 1.3-1.6 M (NH4)2SO4 and 6% V/V glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
