4J5R
TARG1 (C6orf130), Terminal ADP-ribose Glycohydrolase 1 bound to ADP-HPD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.803, 72.004, 81.919 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.170 - 1.250 |
| R-factor | 0.12008 |
| Rwork | 0.118 |
| R-free | 0.15283 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4j5q |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.888 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.270 |
| High resolution limit [Å] | 1.250 | 3.390 | 1.250 |
| Rmerge | 0.076 | 0.036 | 0.402 |
| Number of reflections | 79051 | ||
| <I/σ(I)> | 7.6 | ||
| Completeness [%] | 94.3 | 94.8 | 64.6 |
| Redundancy | 5 | 5.2 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 100mM Imidazole, 20% PEG3350, 200 mM NaCl , pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
