4J50
Crystal Structure of an Expanded RNA CAG Repeat
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-25 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97937 |
| Spacegroup name | H 3 |
| Unit cell lengths | 46.338, 46.338, 134.597 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.433 - 1.650 |
| R-factor | 0.169 |
| Rwork | 0.169 |
| R-free | 0.17970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.697 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 13022 | |
| Completeness [%] | 99.8 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 15 mM Mg Acetate, 50 mM Na cacodylate, 1.7 M Am Sulfate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
