4J4L
Modular evolution and design of the protein binding interface
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.319, 134.001, 148.722 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.090 - 2.300 |
| Rwork | 0.205 |
| R-free | 0.26843 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1alu |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.796 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.090 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 38083 | |
| Completeness [%] | 95.8 | 78.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 290 | 0.1M magnesium formate, 15-18% w/v polyethylene glycol 3350, pH 8.0, VAPOR DIFFUSION, temperature 290K |
