4J4L
Modular evolution and design of the protein binding interface
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.319, 134.001, 148.722 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.090 - 2.300 |
Rwork | 0.205 |
R-free | 0.26843 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1alu |
RMSD bond length | 0.015 |
RMSD bond angle | 1.796 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.090 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 38083 | |
Completeness [%] | 95.8 | 78.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 290 | 0.1M magnesium formate, 15-18% w/v polyethylene glycol 3350, pH 8.0, VAPOR DIFFUSION, temperature 290K |