4J4J

Crystal structure of the APOBEC3F Vif binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97921
Spacegroup name	I 4 2 2
Unit cell lengths	164.046, 164.046, 135.315
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.490 - 3.100
R-factor	0.2406
Rwork	0.236
R-free	0.27690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.495
Data reduction software	d*TREK (9.9.9.1D)
Data scaling software	d*TREK (9.9.9.1D)
Phasing software	PHASER
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.490	43.490	3.110
High resolution limit [Å]	3.000	6.460	3.000
Rmerge	0.100	0.046	0.838
Total number of observations		19386	19991
Number of reflections	18499
<I/σ(I)>	7.2	26.8	1.3
Completeness [%]	98.5	97.2	100
Redundancy	9.6	9.11	10.45

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	298	18% (w/v) PEG 8000, 0.1 M CHES pH 9.0, 25% (w/v) glucose, VAPOR DIFFUSION, HANGING DROP, temperature 298K