4J4F
Structure of P51G Cyanovirin-N swapped tetramer in the P212121 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2011-11-25 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.907, 74.482, 101.102 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.290 - 1.900 |
| R-factor | 0.23933 |
| Rwork | 0.234 |
| R-free | 0.28441 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ezm |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.280 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.290 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.109 | 0.264 |
| Number of reflections | 34612 | |
| <I/σ(I)> | 9.4 | 1.9 |
| Completeness [%] | 95.0 | 62.5 |
| Redundancy | 5.64 | 2.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | 35% w/v MPD, 0.1 M sodium/potassium phosphate, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
