4J4D
Structure of P51G Cyanovirin-N swapped dimer in the P21212 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-11-10 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 52.313, 62.981, 114.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.310 - 2.000 |
| R-factor | 0.21786 |
| Rwork | 0.215 |
| R-free | 0.26582 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ezm |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.503 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.310 | 1.960 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.072 | 0.396 |
| Number of reflections | 28499 | |
| <I/σ(I)> | 13.3 | 3.5 |
| Completeness [%] | 92.1 | 86.5 |
| Redundancy | 8.75 | 8.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | 40% ethanol, 0.1 M sodium phosphate/citrate, pH 4.2, 5% w/v PEG1000, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
