4J4C
Structure of P51G Cyanovirin-N swapped dimer in the P3221 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-11-12 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 47.928, 47.928, 78.815 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.730 - 1.900 |
| R-factor | 0.19539 |
| Rwork | 0.194 |
| R-free | 0.22730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ezm |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.289 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.730 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.433 |
| Number of reflections | 8659 | |
| <I/σ(I)> | 18.6 | 5 |
| Completeness [%] | 99.6 | 98.1 |
| Redundancy | 14.28 | 14.17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | 20% w/v PEG8000, 0.1 M sodium phosphate/citrate, pH 4.2, 0.2 M sodium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
