4J34
Crystal Structure of kynurenine 3-monooxygenase - truncated at position 394 plus HIS tag cleaved.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-19 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.070, 98.780, 86.300 |
| Unit cell angles | 90.00, 105.77, 90.00 |
Refinement procedure
| Resolution | 56.800 - 2.030 |
| R-factor | 0.2032 |
| Rwork | 0.201 |
| R-free | 0.24110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 56.800 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 60736 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | Crystals were grown by mixing 200 nl of protein (buffer A) with 200 nl of a reservoir solution containing 0.1 M imidazole, pH 7.8, and 11 % w/v polyethylene glycol 8K , VAPOR DIFFUSION, SITTING DROP, temperature 277K |
