4J26
Estrogen Receptor in complex with proline-flanked LXXLL peptides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-06-05 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 |
| Unit cell lengths | 62.630, 62.630, 125.670 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.600 - 2.300 |
| R-factor | 0.2051 |
| Rwork | 0.203 |
| R-free | 0.24197 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3oll |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.247 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.600 | 2.600 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.167 | 0.273 |
| Number of reflections | 24203 | |
| <I/σ(I)> | 14.93 | 5.24 |
| Completeness [%] | 98.8 | 99.4 |
| Redundancy | 2.35 | 1.89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 277 | 10%-20% PEG6000, 0.6M-2M LiCl, 100mM Citric Acid, pH 5.0, VAPOR DIFFUSION, temperature 277K |
