4J26
Estrogen Receptor in complex with proline-flanked LXXLL peptides
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-06-05 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 |
Unit cell lengths | 62.630, 62.630, 125.670 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.600 - 2.300 |
R-factor | 0.2051 |
Rwork | 0.203 |
R-free | 0.24197 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3oll |
RMSD bond length | 0.009 |
RMSD bond angle | 1.247 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.600 | 2.600 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.167 | 0.273 |
Number of reflections | 24203 | |
<I/σ(I)> | 14.93 | 5.24 |
Completeness [%] | 98.8 | 99.4 |
Redundancy | 2.35 | 1.89 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5 | 277 | 10%-20% PEG6000, 0.6M-2M LiCl, 100mM Citric Acid, pH 5.0, VAPOR DIFFUSION, temperature 277K |