4J1B

X-ray structure of the adduct between hen egg white lysozyme and AziRu (black crystal)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	78.009, 78.009, 37.192
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.570 - 1.660
R-factor	0.187
Rwork	0.187
R-free	0.24600
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.022
RMSD bond angle	2.100
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.660
Number of reflections	13847
Completeness [%]	97.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	1.0-1.4 M NaCl, 0.050 M acetate buffer.Crystals were soaked in a saturated solution of AziRu containing 2.4 M NaCl in the same buffer. After a few hours, crystals changed their colour to yellow and then green. In a few days they turned to black, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K