4J18

Crystal structure of H191L mutant of UDP-glucose pyrophosphorylase from Leishmania major

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-11-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91985
Spacegroup name	P 43
Unit cell lengths	101.310, 101.310, 71.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.500 - 2.350
R-factor	0.18075
Rwork	0.178
R-free	0.23221
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2oef
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.500	2.400
High resolution limit [Å]	2.350	2.350
Number of reflections	30206
<I/σ(I)>	9.8
Completeness [%]	99.2	98.2
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	293.15	10% v/v glycerol, 0.05 M citrate buffer, 1.1 M sodium citrate , pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K