4J15
Crystal structure of human cytosolic aspartyl-tRNA synthetase, a component of multi-tRNA synthetase complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 300 |
| Detector technology | CCD |
| Collection date | 2013-01-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.885, 141.921, 68.495 |
| Unit cell angles | 90.00, 102.19, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.240 |
| R-factor | 0.19876 |
| Rwork | 0.197 |
| R-free | 0.22775 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1asz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.290 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.290 |
| High resolution limit [Å] | 2.240 | 2.250 |
| Number of reflections | 48428 | |
| Completeness [%] | 99.3 | 95.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 20% PEG3350, 8% tacsimate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
