4J0U

Crystal structure of IFIT5/ISG58

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9798
Spacegroup name	P 21 21 21
Unit cell lengths	63.722, 71.529, 115.527
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.725 - 1.969
R-factor	0.1956
Rwork	0.193
R-free	0.23650
Structure solution method	SAD
RMSD bond length	0.021
RMSD bond angle	1.038
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.000
High resolution limit [Å]	1.969	5.350	1.970
Number of reflections	37930
Completeness [%]	99.0	98.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	289	100mM HEPES, 150mM NaCl, 8% PEG3350, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 289K
1	VAPOR DIFFUSION, HANGING DROP	6.9	289	100mM HEPES, 150mM NaCl, 8% PEG3350, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 289K