4J0Q

Crystal structure of Pseudomonas putida elongation factor Tu (EF-Tu)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-10-23
Detector	Pilatus 6M-F
Wavelength(s)	0.976300
Spacegroup name	C 1 2 1
Unit cell lengths	212.505, 155.624, 99.298
Unit cell angles	90.00, 114.76, 90.00

Refinement procedure

Resolution	48.242 - 2.294
R-factor	0.1742
Rwork	0.174
R-free	0.21290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4iw3
RMSD bond length	0.010
RMSD bond angle	1.254
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.380
High resolution limit [Å]	2.294	4.950	2.300
Rmerge	0.056	0.041	0.826
Number of reflections	129510
<I/σ(I)>	10.3
Completeness [%]	99.3	99.6	93.9
Redundancy	6.6	6.7	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	277	0.1M MgOAc, 20% MPD, 0.05M MES pH 5.6, 10mM SrCl2, VAPOR DIFFUSION, SITTING DROP, temperature 277K