4J0Q
Crystal structure of Pseudomonas putida elongation factor Tu (EF-Tu)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-23 |
| Detector | Pilatus 6M-F |
| Wavelength(s) | 0.976300 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 212.505, 155.624, 99.298 |
| Unit cell angles | 90.00, 114.76, 90.00 |
Refinement procedure
| Resolution | 48.242 - 2.294 |
| R-factor | 0.1742 |
| Rwork | 0.174 |
| R-free | 0.21290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4iw3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.254 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.5.2) |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
| High resolution limit [Å] | 2.294 | 4.950 | 2.300 |
| Rmerge | 0.056 | 0.041 | 0.826 |
| Number of reflections | 129510 | ||
| <I/σ(I)> | 10.3 | ||
| Completeness [%] | 99.3 | 99.6 | 93.9 |
| Redundancy | 6.6 | 6.7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 0.1M MgOAc, 20% MPD, 0.05M MES pH 5.6, 10mM SrCl2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
