4J0Q
Crystal structure of Pseudomonas putida elongation factor Tu (EF-Tu)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-10-23 |
Detector | Pilatus 6M-F |
Wavelength(s) | 0.976300 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 212.505, 155.624, 99.298 |
Unit cell angles | 90.00, 114.76, 90.00 |
Refinement procedure
Resolution | 48.242 - 2.294 |
R-factor | 0.1742 |
Rwork | 0.174 |
R-free | 0.21290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4iw3 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.254 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.5.2) |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
High resolution limit [Å] | 2.294 | 4.950 | 2.300 |
Rmerge | 0.056 | 0.041 | 0.826 |
Number of reflections | 129510 | ||
<I/σ(I)> | 10.3 | ||
Completeness [%] | 99.3 | 99.6 | 93.9 |
Redundancy | 6.6 | 6.7 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 0.1M MgOAc, 20% MPD, 0.05M MES pH 5.6, 10mM SrCl2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |